DELS in Cells - Snap
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– partner with Vipergen
DELs in Cells – Snap
You provide your target amino acid sequence, and we provide DEL screening inside a living cell in two stages.
- Never been easier
- Pay as you go – two payments
- Low cost
- Standardized process
- High success rate
- No need for purified target protein
- Screen under physiologically relevant conditions
- Hit exclusivity
- You provide the amino acid sequence of your target protein
- We test for successful protein expression
- You receive report & go-no-go recommendation
- Timeline: 1 month
- You say “Go”
- We screen your target in a living cell with 500 million member high diversity/high quality DEL
- You receive report and chemical structure information for 25 top hits
- Timeline: 1 month
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Business Models
| DELs in Cells | DELs in Cells – Snap | |
|---|---|---|
| Input | target protein sequence | target protein sequence |
| Cost | $$ (quotation) | $ (fixed price) |
| Target protein size | up to 500 kDa | up to 75 kDa |
| Integral membrane proteins amenable | yes (cytoplasmic side) | yes (cytoplasmic side) |
| Purified target protein requirement | no | no |
| DNA-encoded library (truncates eliminated) | >1 billion | ~500 million |
| Assay development requirement | no | no |
| Screening design | customized | predefined |
| Assay development requirement | no | no |
| TAT | 8-20 weeks | 8 weeks |
| Deliverables | structures of all hits | structures of up to 25 hits |
| Hit exclusivity | yes | yes |
Available DNA Encoded Libraries
| Lib047 | Lib057 | Lib081 | |
|---|---|---|---|
| Size (million compounds) | 499 | 535 | 381 |
| Molecular weight (avg) | 605 | 525 | 525 |
| cLogP (avg) | 2.7 | 0.77 | 0.9 |
| Rotatable bonds (avg) | 10.5 | 8.7 | 8.9 |
| TPSA (avg) | 152 | 138 | 137 |
| Fsp3 (avg) | 0.5 | 0.6 | 0.6 |
Characteristics of Vipergen’s DNA Encoded Libraries
- Low false positive rate
- High fidelity – 100% code to compound correspondence
- Truncates eliminated
- Only robust chemistries
- Diversity by building blocks
- Resynthesized hits (off-DNA) available from us
Frequently Asked Questions
What is a DEL?
DNA Encoded Libraries are collections of small molecules, each linked to unique DNA sequences. These DNA sequences act as barcodes for the small molecules, allowing the tracking of the individual small molecules. The use of DELs facilitates rapid screening and identification of hits from large chemical libraries, streamlining the drug discovery process.
Cells used for screening
Xenopus laevis oocytes, often referred to as the “living test tube,” have been widely used in research since the 70s. They are large cells, approximately 1 mm in diameter with a volume of about 1 µL, which allows for microinjections. They are highly suitable for conducting individual experiments within a single cell, often described as one cell, one experiment. The oocytes are particularly effective for expressing heterologous proteins, achieving a success rate of over 95%. This makes them a valuable tool in various fields of research, especially when studying the function and pharmacology of proteins in a living cell environment.
Is purified protein necessary to initiate DEL screening in cells?
No, unlike other screening technologies, only the amino acid sequence of the target protein is needed to execute the screening project, enabling a rapid progression from project initiation to hits that provides our partners with a competitive edge.
How is the expression of the target protein in the cell validated?
The target protein expression is ensured by Western blotting, a rapid technology enabling semi-quantitative and qualitative assessment of the expressed protein’s integrity.
Which target classes can be screened in cells?
The screening of most cytoplasmic proteins, including membrane proteins accessible from the cytoplasm, can be conducted in cells. Various protein classes, encompassing enzymes, protein-protein interaction targets, molecular glue targets, and many more have been screened in this format.
How is diversity and novelty ensured in the DNA encoded libraries?
Diversity and novelty are assured by the diversity of the building blocks incorporated into the design of our libraries. A substantial portion of the building blocks, used in library synthesis, is designed in-house. This approach ensures the novelty of the resulting compounds in the library.