High fidelity drug discovery - Get an early lead
Vipergen presents three technologies for DNA encoded libraries (DEL) which together deliver the highest fidelity drug discovery capabilities. The YoctoReactor® (yR) technology creates vast small molecule DELs in a single tube. Binder Trap Enrichment® (BTE) is a homogeneous screening technology for rapid and comprehensive screening of DELs. Cellular Binder Trap Enrichment® (cBTE) is a technology for screening DELs inside a living cell.
Key features of yR/BTE and yR/cBTE high fidelity drug discovery
YoctoReactor® – Technology platform for synthesizing DNA-encoded chemical libraries
yoctoReactor® (yR) libraries containing hundreds of millions of DNA-encoded drug-like small molecules are synthesized simultaneously in a single tube approach by drawing on the self-assembly of complementary DNA into DNA junctions. Chemical building blocks are brought into close proximity facilitating chemical reaction and the attached DNA ultimately encodes the final product. The overall design has favorable implications for the scope and reliability of the chemistry, the stability of the structure, and, ultimately, the capability for addressing the most challenging targets.
- 100% match between the DNA-code and the synthesized small molecule is ensured by purification steps after each chemical reaction (unique capability) and use of robust chemistries
The yR is shown here as space-filling model of a DNA 4-way junction. The volume of the reactor is on the order of a yoctoliter (10-24 liter). It can therefore facilitate and control single-molecule reactions.
Binder Trap Enrichment® – Technology platform for DEL screening in a homogeneous assay
Binder Trap Enrichment® (BTE) is a homogeneous screening methodology for yR libraries which identifies small molecule binders to a protein of interest. A large subset of these binders are found to be biologically active hits.
- As a homogeneous assay, BTE delivers high fidelity and a low false positive rate by avoiding surface artifacts and the complexity of matrix binding
- Instant specificity – multiplexed screening of target and anti-targets (unique capability)
Binder Trap Enrichment is a homogeneous screening method for DNA-encoded libraries. Ligands are identified by trapping binding pairs (DNA-labeled target protein and DNA-encoded small molecule) in emulsion droplets during dissociation dominated kinetics. Once trapped, the DNA is joined by ligation, thus preserving the binding information and permits analysis by high capacity DNA sequencing.
Cellular Binder Trap Enrichment® – Technology platform for DEL screening in a living cell
Cellular Binder Trap Enrichment® (cBTE) is an in vivo screening methodology for DEL which identifies small molecule binders to a protein of interest. A large subset of these binders are found to be biologically active hits.
- As an in vivo assay, cBTE delivers lower attrition rate (unique capability)
- No need for purified active target protein – cBTE accommodates a broader target space (unique capability)
