DELs in Cells Express: Confirmed Hits in 30 Business Days
DELs in Cells – Express service is fast and easy. You simply provide the amino acid sequence of your target protein, and Vipergen handles the rest and ships hit compounds to you in just 30 business days. The process is designed for speed without compromising success rate and false positive rate. The utilized DNA encoded library (DEL) is optimized for fast off-DNA hit resynthesis. This makes the DELs in Cells – Express service an excellent choice for projects seeking rapid progression, even if purified target protein is not readily accessible.
Summary
DELs in Cells – Express takes you from a protein sequence to synthesized hits in as little as 30 business days. No purified protein is needed from your end.
How fast: 30 business days, in three clear stages: 10 for the expression study, 10 for screening, and 10 for off-DNA resynthesis of your selected hits.
What you provide: Just the amino acid sequence of your target. Screening runs in living cells, so no purified protein is required.
What you get: A hit list with screening metrics plus chemical structures ready for confirmation.
What you keep: You own all foreground IP, data, and hits for the chemical series that you choose to nominate with no downstream financial obligations.
Service in brief
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1. You provide the amino acid sequence of the target protein(s)
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2. We conduct an expression study and provide a report
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3. You say ”go”
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4. We conduct the screening, perform the analysis and provide a report, including hit list
with chemical structure information -
5. You order hits
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6. We synthesize the hits and ship to you
Timelines
Expression study: 10 business days (for target with MW up to 75 kDa)
Screening: 10 business days
Resynthesis of hits: 10 business days (for up to 25 hits per batch)
Keys
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Screen is conducted in a living cell -
More physiologically relevant conditions
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Lower attrition rate (assumed)
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Broader target space – no need for purified target protein
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Higher success rate (>80%)
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Shorter TAT
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Identifies hits and hit families
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Low false positive rates (100% match between DNA code and compound)
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Applicable across disease areas and target classes (no structural information required)
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Demonstrated ability to address targets considered difficult, including transcription factors, protein complexes, and PPI targets
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Straight forward and transparent data analysis
Business Model
- Simple fee-based plan with no reach-through and royalty payments
- Hit exclusivity and transparent cost structure – Client owns all hits and foreground IP without any downstream financial obligations
- The compensation plan comprises a total of 3 payments as a transparent cost structure
- Pre-Project Expression Study Fee – scales with work
- Screening Fee – scales with screening slots
- Chemical Series Nomination Fee
- The project flow is rapid and straightforward:
- Client provides the amino acid sequence for the target protein
- Vipergen performs the Expression Study (go-no-go)
- Vipergen performs one round of screenings with varying constructs and different libraries.
- Vipergen delivers a hit list and report, including metrics from screens and chemical structure information
- Hits are reserved for client for 1 year
- Client may resynthesize and test hits in relevant assays and use data for 1 year
- Vipergen can assist in off-DNA resynthesis as a fee-for-service
- Client may nominate Chemical Series for 1 year
- Vipergen assigns its rights to Nominated Chemical Series, and these become exclusive for you as a client
- Client own all foreground IP, data and hits of nominated series without royalty payment or other downstream financial obligations
DELs
Vipergen’s high fidelity DELs are synthesized using robust chemistry and with a purification step after each chemical transformation, thus providing 100% correspondence between code and compound (no truncates). DELs applied for the Express service are optimized for fast off-DNA hit resynthesis.
Key drivers for library design
- Chemical diversity
- Physicochemical properties
- Fidelity
- Hit resynthesis (off DNA)
Target classes
- Kinases
- Oxidoreductase Proteases
- Hydrolases
- Isomerases
- Transferases
- Lyases
- Phosphatases
- Transcription factors
- Synthases
- E3 biquitin ligases
- Deubiquitinases (DUBs)
- Helicases
- Polymerases
- Nucleotide exchange factors
- Decarboxylases
- Ligases
- Integral membrane proteins
- G protein-coupled receptors (GPCRs)
- Ion channels
- Single-pass membrane proteins
- Nuclear receptors
- Cytosolic receptors
- Extracellular receptors
- Transcription factors
- E3 ligases
- Deubiquitinases (DUBs)
- Antibodies
- Cytokines
- Interleukins
- Growth factors
- Carrier proteins
- Adaptor proteins
- Oncoproteins
- Histone binders
- Methyl transferases
- Acetyltransferase
- Demethylases
- Deacetylases
- Viral proteins (enzymes and coat proteins)
- Bacterial proteins (enzymes and receptors)
- Fungal proteins (enzymes and receptors)