Questions & Answers
DNA Encoded libraries (DEL)
What is a DNA Encoded Library (DEL)?
A DEL is a collection of small druglike molecules where each compound is attached to a piece of DNA that functions as a barcode for the compound. Thus, each compound has a unique DNA sequence that can be used for identifying it.
Why are DELs significant in drug discovery?
DELs are significant in drug discovery because they provide an efficient way to screen very large compound libraries for new druglike molecules.
How does DEL screening work?
DEL screening works by identifying molecules in the DEL that binds to a drug target. Vipergen uses a proprietary process, Binder Trap Enrichment, to do the binding in solution and avoid matrix effects that are often seen when solid phase binding is employed. In the newest version, the binding is performed in the inside of a living cell. The molecules binding to the target are identified by Next Generation Sequencing.
What are the advantages of using DELs over traditional compound libraries?
The primary advantage of using DELs is that they provide a highly efficient way of screening very large compound libraries in a short time.
Can molecular glue compounds be identified from DELs?
Yes, Vipergen has successfully identified molecular glue compounds from DELs.
CELL-based DEL screening
What is Cell-Based DEL Screening?
In Cell-Based DEL screening, Vipergen uses a proprietary process, where the target protein is expressed in a living cell. The DEL is then injected into the cell, and library compounds binding to the drug target are subsequently identified by DNA sequencing.
How does Cell-Based DEL Screening differ from traditional DEL Screening?
Cell-based DEL screening differs from traditional DEL screening by being performed in the inside of a living cell. The Protein of Interest (the target) is expressed in the cell, and thus, unlike other approaches, purified protein is not needed.
Apart from being faster than traditional approaches, lower attrition rates can also be expected because the screening is performed in the more physiologically relevant context of a living cell.
How do I know that my protein can be screened in cells?
Before screening, Vipergen performs a feasibility study to ensure that the target is expressed appropriately. In our experience, most cytoplasmic proteins, including membrane proteins accessible from the cytoplasm, can be screened in cells.
What protein classes can be screened in cells?
Most cytoplasmic proteins, including membrane proteins accessible from the cytoplasm, can be screened in cells. Vipergen has experience with several protein classes, including enzymes, protein-protein interaction targets, and many more.
Can multimeric proteins be screened in cells?
Yes, Vipergen has successfully screened heteromultimers up to a size of 2.6 MDa in cells. In principle, there is no theoretical limit to the size or complexity of heteromultimer that can be screened in cells.
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What we do
Vipergen revolutionizes drug discovery by screening for novel molecules directly in living cells, providing the tool to target complex molecular entities, improve hit rates, and develop effective pharmaceuticals.
Our approach prioritizes physiological relevance, ensuring a higher success rate and lower attrition rates in drug development.
We excel in finding the right molecular keys for a variety of complex biological locks.
How we do it
Our advanced technology allows us to conduct screenings in living cells, increasing the target space and reducing turnaround time significantly.
Unlike traditional methods, our process does not require a purified version of the target protein, enabling us to handle even the most challenging targets with ease and to initiate screenings more rapidly.
Who we are
Vipergen stands as a pioneer in the biotech and pharmaceutical industry, driven by a commitment to innovation and excellence.
Our team of highly qualified professionals brings together extensive expertise in chemistry and biology.
We are recognized for our unique ability to address complex drug discovery challenges that are beyond the reach of conventional approaches.