Vipergen ApS
Gammel Kongevej 23A
1st floor
1610 Copenhagen
Denmark
VAT: DK28851758
BTE is a homogeneous screening method for DELs which uses water-in-oil emulsion technology to isolate individual ligand-target complexes.
Ligands to a protein target are identified by trapping binding pairs (DNA-labelled protein target and DEL ligand) in emulsion droplets during dissociation dominated kinetics. Once trapped, the target and ligand DNA are joined by ligation, thus preserving the binding information.
Hereafter, BTE is essentially a counting exercise: information on binding events is deciphered by sequencing and counting the joined DNA – selective binders are counted with a higher frequency than random binders. This is possible because random trapping of target and ligand is “diluted” by the high number of compartments in the emulsion.